This book collects and focuses on commonly used and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells. It presents clear, concise information that is immediately useful and applicable. Each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. Each chapter includes a comprehensive list of suppliers for all equipment used in the protocols presented, with complete contact information for the suppliers in a special appendix at the end of the book. Culture of Human Stem Cells

Preface.
List of Contributors.

Abbreviations.

1. Quality Control Procedures for Stem Cell Lines (Glyn N. Stacey and Jonathan M. Auerbach).

2. Human Embryonal Stem Cell Lines: Derivation and Culture (Jessica A. Cooke and Stephen L. Minger).

3. Techniques for Neural Differentiation of Human EC and ES Cells (Jamie P Jackson, Peter D Tonge and Peter W Andrews).

4. Cardiomyocyte Differentiation in Human ES Cells (Christine Mummery).

5. Culture of the Human Germ Cell Lineage (Lee Turnpenny and Neil A Hanley).

6. Derivation and Culture of Human Embryonal Carcinoma Stem Cell Lines (Stefan Przyborski).

7. Culture of Umbilical Cord- and Cord Blood-Derived Stem Cells (Young-Jin Kim).

8. Multipotent Stem Cells in Dental Pulp (Wataru Sonoyama, Takayoshi Yamaza, Stan Gronthos, and Songtao Shi).

9. Fundamentals of Culture and Characterization of Mesenchymal Stem/Progenitor Cells (MSCs) from Bone Marrow Stroma (Carl A. Gregory and Darwin J. Prockop).

10. Isolation, Characterization, and Culture of Soft Connective Tissue Stem/Progenitor Cells (Charles W Archer, Sarah Oldfield, Samantha Redman, Laura Haughton, Gary Dowthwaite, Ilyas Khan and Jim Ralphs).

11. Culture of Human Corneal Stem Cells (Yiqin Du and James L. Funderburgh).

12. Culturing Mammary Stem Cells (Mark A. LaBarge, Ole W. Petersen, and Mina J. Bissell).

13. Tissue Culture of Adipose-Derived Stem Cells (Kristine M. Safford and Henry E. Rice).

Suppliers.

Index.